In contrast, mitochondrial fusion is completely inhibited by protonophores that dissipate the inner membrane potential. This process is maintained in the absence of a functional respiratory chain, despite disruption of microtubules or after significant reduction of cellular ATP levels. Using green and red fluorescent proteins targeted to the mitochondrial matrix, we show that mitochondrial fusion in human cells is efficient and achieves complete mixing of matrix contents within 12 h. Mitochondrial fusion remains a largely unknown process despite its observation by live microscopy and the identification of few implicated proteins.
0 Comments
Leave a Reply. |